16s rRNA/DNA, 28s rRNA/DNA, ITS Sequencing, Identification for bacteria

BioAxis DNA Research Centre Pvt. Ltd. is pioneer in 16s rRNA/DNA, 28s rRNA/DNA, ITS Sequencing, Identification for bacteria and other Microbial identification services needed for research and Industrial purposes in diversified fields of Microbiology like food, dairy, soil, environmental, agricultural and pharmaceutical industries. Our well equipped Microbiology research laboratory offers 16s rRNA/DNA, 28s rRNA/DNA, ITS Sequencing, Identification for bacteria, fungi, algae and yeast by biochemical analytical methods followed by identification based on molecular level. BDRC utilizes the most advanced techniques and methods developed by eminent Scientists having a strong knowledge of the Scientific techniques that satisfies the needs of the clients. Services provided at BDRC are cost effective, most reliable, guaranteed turnaround time based on growth of organism and defensible data.

Warm wishes to our clients, BDRC is continuously growing while still providing 10 years of excellence in microbiology. We wish to go forward with your support to extend our services.

16s rRNA/DNA, 28s rRNA/DNA, ITS Sequencing, Identification for bacteria by DNA Sequencing techniques is the most accurate and reliable method used at BDRC for Microbial identification. Not only the DNA sequencing but BDRC here is providing the identification of bacteria, actinomycetes, fungi and yeasts based on FAME analysis and biochemical characterizations.

List of Microbial DNA Identification Services – 16s rRNA/DNA, 28s rRNA/DNA, ITS Sequencing, Identification for bacteria

16s rRNA/DNA, 28s rRNA/DNA, ITS Sequencing, Identification for bacteria 16s rDNA/28s rDNA/ITS-based microbial identification for bacteria, actinomycetes,fungi and yeasts

Fully validated library entries for bacteria, actinomycetes, yeasts, and fungi

Evolutionary studies of microbes by Biochemical/16s rDNA/FAME/Phylogenetic
analysis

Sample comparison to GenBank, an international DNA sequence database

Bacterial Identification Service

Once isolated and purified, identification of the bacteria is an important aspect of research for the scientists. Checking the authenticity of cultures from time to time is also of major importance as contamination of stock cultures is common. We follow16S rDNA- based protocol for identification of bacteria.

Identification is guaranteed up to genus level while species as well as strain identification would be possible.

Features:

  • Based on 1500 bp of 16S-rDNA gene sequence analysis
  • Identification based on 10 closest homolog bacterial sequence available in NCBI, gene
  • Similarity matrix as well as phylogenetic tree provided
  • Fast and reliable service

Deliverables:

  1. Final report containing the bacterial identification
  2. Similarity Matrix table indicating the homology within 10 most similar
  3. Phylogenetic tree indicating evolutionary relationship of the bacteria with 10 nearest

Delivery Time:

Up to 4 samples: 4 weeks

 Actinomycetes Identification Service

Identification of actinomycetes by traditional methods like biochemical assays is laborious, time consuming and requires expertise. These methods have not always been reproducible and adequate for the identification of newer species of actinomycetes. A rapid PCR-based method for identifying filamentous actinomycete genera is developed based on 16S rDNA to identify actinomycete isolates. Identification is guaranteed up to genus level while species identification would be possible.

Features:

  • Based on 1500 bp of 16S rDNA gene sequence
  • Identification will be based on 10 closest homolog actinomycetes sequence available in NCBI
  • Similarity matrix and phylogenetic tree will be
  • Fast & reliable

Deliverables:

  1. Final report containing the actinomycetes identification infor- mation.
  2. Similarity Matrix table indicating the homology with 10 most similar
  3. Phylogenetic tree indicating evolutionary relationship of the bacteria with 10 nearest neighbors

Delivery Time:

Up to 4 samples: 4 weeks

Fungi Identification Service

 The conventional methods of detection and identification of fungi have mainly relied on culture isolation and subsequent observations of morphological traits. These methods are time consuming, laborious, and may require days to weeks for isolation by culture. In addition, not all the fungal species are cultural on a given medium, which leads to analysis that may not accurately reflect the true fungal community in a sample.

With the advent of polymerase chain reaction (PCR), inexpensive DNA sequencing, and a relatively large databank of ribosomal DNA sequences, it is now possible to more objectively characterize and identify fungal species. However, due to the immense diversity in fungi, true group- and species-specific detection can be difficult to achieve. We apply PCR-based detection techniques of sequence polymorphisms in the internal transcribed spacer 1 & 2 (ITS- 1 & 2) region of the rDNA genes as a means of fungi identification. Unique, PCR products ranging from ~ 690-1300 bp are specific to fungi. Identification is guaranteed up to genus level while species as well as sub species identification would be possible.

Features:

  • Clearly characterize the fungi comparing with larger number of isolates had been utilized in earlier
  • Characterization will be based on ~ 690-1300 bp of ITS1&2 regions of the rRNA genes sequence analysis
  • Identification will be based on 10 closest homolog fungi sequence available in NCBI GenBank
  • Rapid and reliable service for identification of fungi, including potentially new or emerging pathogenic

Deliverables:

  1. Final report containing the fungi identity
  2. Nucleotide sequence percentage homology table indicating the identity within 10 most similar neighbours
  3. Phylogenetic tree displaying evolutionary relationships among the 10 nearest neighbours fungal species included in the

Delivery Time:

6 Weeks

Yeast Identification Service

The identification and classification of yeasts have traditionally been based on morphological, physiological and biochemical traits. Identification of relevant yeasts can be time-consuming and inaccurate with most of the available methods. We use PCR-based detection of sequence polymorphisms in the internal transcribed spacer 2 (ITS2) region of the rDNA genes as a means of yeast identification. Unique, PCR products ranging from ~ 230-450 bp are specific to yeast. These data, and the specificity of length polymorphisms for identifying yeasts, are confirmed by DNA sequence analysis of the ITS2 region. DNA Sequences specific to species can be easily distinguished by restriction enzyme analysis if needed.

Identification is guaranteed up to genus level while species as well as strain identification would be possible.

Features:

  • Based on ~ 230-450 bp of ITS2 region of the rDNA gene se- quence analysis
  • Restriction analysis of PCR-amplified ITS2 region DNA if required
  • Identification will be based on 10 closest homolog yeast sequence available in NCBI GenBank
  • Rapid and reliable method to identify yeasts, including potentially new or emerging pathogenic

Deliverables:

  1. Final report containing the yeast identity
  2. Nucleotide sequence percentage homology table indicating the identity within 10 most similar neighbours
  3. Phylogenetic tree displaying evolutionary relationship of the yeast with 10 nearest neighbours

Delivery Time: 4-6 weeks.